indirect immunofluorescence
- indirect immunofluorescence
-
A method of
immunofluorescence staining in which the first antibody, that is directed against the
antigen to be localized, is used unlabelled, and the location of the first
antibody is then detected by use of
a fluorescently labelled anti-IgG (against IgGs of the species in which the first
antibody was raised). The advantage is that there is some amplification, and
a well-characterized goat anti-rabbit
IgG antibody can, for example, be used against
a scarce specific
antibody raised in rabbits. The same technique can be used for ultrastructural localization of the first
antibody by substituting
peroxidase or gold-labelled second antibody.
Dictionary of molecular biology.
2004.
Look at other dictionaries:
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indirect — in·di·rect .in də rekt, dī adj 1) not direct: as a) not resulting directly from an action or cause (as a disease) <indirect effects of malaria> b) involving intermediate or intervening parts or pathways <stimulation of one eye elicits… … Medical dictionary
indirect fluorescent antibody test — see immunofluorescence … Medical dictionary
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ИММУНОФЛЮОРЕСЦЕНЦИЯ — (immunofluorescence) метод определения количества и/или распределения какого либо антитела или антигена в тканевом срезе. При этом антитела маркируются (прямо или косвенно) с помощью флюоресцентного красителя (например, флюоресцина) и затем… … Толковый словарь по медицине
Chromatin bridge — Classification and external resources DAPI staining allows for visualization of deoxyribonucleic acid portions of the two daughter cells. The thin “string like” DNA connecting them is defined as a chromatin bridge. Chromatin bridge is a mitotic… … Wikipedia
Pathogenic bacteria — Clostridium tetani Bacterial infection Classification and external resources MeSH … Wikipedia
